Journal:

Article Title: MurF Inhibitors with Antibacterial Activity: Effect on Muropeptide Levels ▿

doi: 10.1128/AAC.00166-09

Figure Lengend Snippet: Relative amounts of UDP-MurNAc-tripeptide and -pentapeptide in E. coli OC2530 overexpressing MurF a

Article Snippet: In contrast, cycloserine was more potent than DQ1 against wild-type E. coli (MICs, 16 to 32 μg/ml) and displayed activity (MICs, 32 to 128 μg/ml) against the strains of gram-positive bacteria tested. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 1. caption a7 Structures of the MurF inhibitors DQ1 and DQ2. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain MIC (μg/ml) DQ1 DQ1 plus 2 μg/ml PMBN PMBN only DQ2 Cycloserine E. coli OC2530 (LPS defective) 8 2 16 8 64 E. coli OC2605 >32 4 32 >32 16 E. coli OC9040 >32 8 64 >32 32 E. coli ATCC 25922 >32 8 64 >32 32 E. faecalis ATCC 29212 >32 >32 >64 8 128 E. faecium OC3312 >32 >32 >64 8 64 S. aureus ATCC 29213 >32 >32 >64 8 32 Methicillin-resistant S. aureus OC3726 >32 >32 >64 8 32 Methicillin-resistant S. aureus OC2878 >32 >32 >64 8 64 S. aureus OC4172 >32 >32 >64 8 32 Open in a separate window Susceptibilities of bacterial strains to MurF inhibitors DQ1 and DQ2 The hydrophobic nature of DQ1 suggested that poor permeability might be responsible for the lack of measureable MICs for wild-type E. coli .

Techniques: Control

Journal:

Article Title: MurF Inhibitors with Antibacterial Activity: Effect on Muropeptide Levels ▿

doi: 10.1128/AAC.00166-09

Figure Lengend Snippet: Susceptibilities of bacterial strains to MurF inhibitors DQ1 and DQ2

Article Snippet: In contrast, cycloserine was more potent than DQ1 against wild-type E. coli (MICs, 16 to 32 μg/ml) and displayed activity (MICs, 32 to 128 μg/ml) against the strains of gram-positive bacteria tested. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 1. caption a7 Structures of the MurF inhibitors DQ1 and DQ2. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain MIC (μg/ml) DQ1 DQ1 plus 2 μg/ml PMBN PMBN only DQ2 Cycloserine E. coli OC2530 (LPS defective) 8 2 16 8 64 E. coli OC2605 >32 4 32 >32 16 E. coli OC9040 >32 8 64 >32 32 E. coli ATCC 25922 >32 8 64 >32 32 E. faecalis ATCC 29212 >32 >32 >64 8 128 E. faecium OC3312 >32 >32 >64 8 64 S. aureus ATCC 29213 >32 >32 >64 8 32 Methicillin-resistant S. aureus OC3726 >32 >32 >64 8 32 Methicillin-resistant S. aureus OC2878 >32 >32 >64 8 64 S. aureus OC4172 >32 >32 >64 8 32 Open in a separate window Susceptibilities of bacterial strains to MurF inhibitors DQ1 and DQ2 The hydrophobic nature of DQ1 suggested that poor permeability might be responsible for the lack of measureable MICs for wild-type E. coli .

Techniques:

Journal:

Article Title: MurF Inhibitors with Antibacterial Activity: Effect on Muropeptide Levels ▿

doi: 10.1128/AAC.00166-09

Figure Lengend Snippet: (A) Growth curves of E. coli OC2530 treated with DQ1. DQ1 was added to mid-logarithmic-phase cultures at 0.5× MIC (diamonds), 1× MIC (circles), 2× MIC (triangles), or 4× MIC (solid squares); and the OD was monitored. The growth curves of a control (to which DMSO was added; solid line) and a cycloserine-treated culture (2× MIC; open squares) are also shown. Aliquots of the cultures were removed for quantitation of CFU at 30 min (B) and 3 h (C).

Article Snippet: In contrast, cycloserine was more potent than DQ1 against wild-type E. coli (MICs, 16 to 32 μg/ml) and displayed activity (MICs, 32 to 128 μg/ml) against the strains of gram-positive bacteria tested. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 1. caption a7 Structures of the MurF inhibitors DQ1 and DQ2. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain MIC (μg/ml) DQ1 DQ1 plus 2 μg/ml PMBN PMBN only DQ2 Cycloserine E. coli OC2530 (LPS defective) 8 2 16 8 64 E. coli OC2605 >32 4 32 >32 16 E. coli OC9040 >32 8 64 >32 32 E. coli ATCC 25922 >32 8 64 >32 32 E. faecalis ATCC 29212 >32 >32 >64 8 128 E. faecium OC3312 >32 >32 >64 8 64 S. aureus ATCC 29213 >32 >32 >64 8 32 Methicillin-resistant S. aureus OC3726 >32 >32 >64 8 32 Methicillin-resistant S. aureus OC2878 >32 >32 >64 8 64 S. aureus OC4172 >32 >32 >64 8 32 Open in a separate window Susceptibilities of bacterial strains to MurF inhibitors DQ1 and DQ2 The hydrophobic nature of DQ1 suggested that poor permeability might be responsible for the lack of measureable MICs for wild-type E. coli .

Techniques: Control, Quantitation Assay

Journal:

Article Title: MurF Inhibitors with Antibacterial Activity: Effect on Muropeptide Levels ▿

doi: 10.1128/AAC.00166-09

Figure Lengend Snippet: Microscopy of E. coli OC2530 incubated for 5 h without DQ1 (A and C) and with 0.5× MIC of DQ1 (B and D). (A and B) light microscopy; (C and D) TEM.

Article Snippet: In contrast, cycloserine was more potent than DQ1 against wild-type E. coli (MICs, 16 to 32 μg/ml) and displayed activity (MICs, 32 to 128 μg/ml) against the strains of gram-positive bacteria tested. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 1. caption a7 Structures of the MurF inhibitors DQ1 and DQ2. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain MIC (μg/ml) DQ1 DQ1 plus 2 μg/ml PMBN PMBN only DQ2 Cycloserine E. coli OC2530 (LPS defective) 8 2 16 8 64 E. coli OC2605 >32 4 32 >32 16 E. coli OC9040 >32 8 64 >32 32 E. coli ATCC 25922 >32 8 64 >32 32 E. faecalis ATCC 29212 >32 >32 >64 8 128 E. faecium OC3312 >32 >32 >64 8 64 S. aureus ATCC 29213 >32 >32 >64 8 32 Methicillin-resistant S. aureus OC3726 >32 >32 >64 8 32 Methicillin-resistant S. aureus OC2878 >32 >32 >64 8 64 S. aureus OC4172 >32 >32 >64 8 32 Open in a separate window Susceptibilities of bacterial strains to MurF inhibitors DQ1 and DQ2 The hydrophobic nature of DQ1 suggested that poor permeability might be responsible for the lack of measureable MICs for wild-type E. coli .

Techniques: Microscopy, Incubation, Light Microscopy

Journal:

Article Title: MurF Inhibitors with Antibacterial Activity: Effect on Muropeptide Levels ▿

doi: 10.1128/AAC.00166-09

Figure Lengend Snippet: Relative amounts of UDP-MurNAc-tripeptide and -pentapeptide at different concentrations of MurF inhibitors DQ1 and DQ2

Article Snippet: In contrast, cycloserine was more potent than DQ1 against wild-type E. coli (MICs, 16 to 32 μg/ml) and displayed activity (MICs, 32 to 128 μg/ml) against the strains of gram-positive bacteria tested. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 1. caption a7 Structures of the MurF inhibitors DQ1 and DQ2. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain MIC (μg/ml) DQ1 DQ1 plus 2 μg/ml PMBN PMBN only DQ2 Cycloserine E. coli OC2530 (LPS defective) 8 2 16 8 64 E. coli OC2605 >32 4 32 >32 16 E. coli OC9040 >32 8 64 >32 32 E. coli ATCC 25922 >32 8 64 >32 32 E. faecalis ATCC 29212 >32 >32 >64 8 128 E. faecium OC3312 >32 >32 >64 8 64 S. aureus ATCC 29213 >32 >32 >64 8 32 Methicillin-resistant S. aureus OC3726 >32 >32 >64 8 32 Methicillin-resistant S. aureus OC2878 >32 >32 >64 8 64 S. aureus OC4172 >32 >32 >64 8 32 Open in a separate window Susceptibilities of bacterial strains to MurF inhibitors DQ1 and DQ2 The hydrophobic nature of DQ1 suggested that poor permeability might be responsible for the lack of measureable MICs for wild-type E. coli .

Techniques: Control

Journal: Journal of Functional Biomaterials

Article Title: FeMn with Phases of a Degradable Ag Alloy for Residue-Free and Adapted Bioresorbability

doi: 10.3390/jfb13040185

Figure Lengend Snippet: Effect of degradation supernatant on the growth of the reference strain Escherichia coli (ATCC ® 25922). Results represent effects caused by the previous degradation in 3 mL Mueller–Hinton broth. Mean value +/− standard deviation from four replicates. The optical density of the untreated control was set as 100%. Repeated measures analysis of variance (ANOVA) summary with Holm–Šídák’s multiple comparisons test in comparison to control; p < 0.001 = * (GraphPad Prism 9.1.0.; GraphPad Software, LLC, San Diego, CL, USA).

Article Snippet: Standard strains of Escherichia coli ( E. coli ; ATCC ® 25922, Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany) are used since these bacteria are common etiologic agents of clinical infections involved in bone tissue infections related to implants.

Techniques: Standard Deviation, Control, Comparison, Software